![]() In summary, our study shows that discus mucus proteome undergoes changes in protein expression during parental-care period.Īlthough fish have been kept for more than three centuries as ornamentals, and the development of manufactured feed since 50 years ago has contributed to the tremendous growth of this hobby, nutrition of ornamental fish is based on extrapolation of results derived from food fishes under intensive farming conditions. A C-type lectin was also found to be uniquely expressed in parental mucus and could have important role in providing antimicrobial defense to both parental fish and fry. Concurrently, the detection of several antioxidant-related proteins such as thioredoxin peroxidase and hemopexin suggests a need to overcome oxidative stress during hypermucosal production in parental-care behavior. MS analysis of these spots identified proteins such as fructose biphosphate aldolase, nucleoside diphosphate kinase, and heat shock proteins, which are essential to support energy provision, cell repair and proliferation, stress mediation, and defense mechanism in parental fish during parental-care period. ![]() In tandem, another 18 spots were uniquely expressed in parental mucus. Gel analysis revealed a total of 35 spots that were up-regulated in parental mucus. We utilized proteomics to compare protein profile from parental and nonparental fish. Both male and female fish utilized epidermal mucus secreted from specialized epidermal cells to feed developing fry. The discus fish (Symphysodon aequifasciata) is a cichlid demonstrating advanced mode of parental care towards fry.
0 Comments
Leave a Reply. |